Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-FLT3 (Tyr591) (54H1) Mouse mAb #3466

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M Transfected Only 130 nonglycosylated form;160 glycosylated mature form Mouse IgG2b

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-FLT3 (Tyr591) (54H1) Mouse mAb detects transfected levels of FLT3 only when phosphorylated at tyrosine 591. The antibody may cross-react with some tyrosine-phosphorylated proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequences surrounding Tyr591 of human FLT3.

Western Blotting

Western Blotting

Western blot analysis of extracts from Baf3/FLT3 cells, untreated or stimulated with FLT-ligand (3 µg/ml for 2 minutes), using Phospho-FLT3 (Tyr591) (54H1) Mouse mAb (upper) or FLT3 antibody lower.

Background

FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).

  1. Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev. 9, 37-48.
  2. Naoe, T. et al. (2001) Cancer Chemother. Pharmacol. 48 Suppl1, S27-S30.
  3. Namikawa, R. et al. (1996) Stem Cells 14, 388-395.
  4. Beslu, N. et al. (1996) J. Biol. Chem. 271, 20075-20081.
  5. Zhang, S. and Broxmeyer, H.E. (2000) Biochem. Biophys. Res. Commun. 277, 195-199.
  6. Zhang, S. et al. (1999) J. Leukoc. Biol. 65, 372-380.
  7. Mizuki, M. et al. (2000) Blood 96, 3907-3914.

Application References

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Companion Products

US Patent No. 7,183,385 and foreign equivalents assigned to Cell Signaling Technology, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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