Cell Signaling Technology

Product Pathways - Metabolism

Perilipin (D418) Antibody #3470

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P IF-IC M (H) Endogenous 62 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Perilipin (D418) Antibody detects endogenous levels of total perilipin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Asp418 of human perilipin. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 and differentiated 3T3-L1 cells using Perilipin (D418) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells, control (left) or differentiated (right), using Perilipin (D418) Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brown fat using Perilipin (D418) Antibody in the presence of control peptide (left) or antigen-specific peptide (right).


IF-IC

IF-IC

Confocal immunofluorescent analysis of differentiated 3T3-L1 cells using Perilipin (D418) Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Triacylglycerol is stored in lipid droplets as a primary energy reserve. Perilipin is localized at the periphery of lipid droplets and serves as a protective coating against lipases (1-3). Evidence suggests that PKA regulates lipolysis by phosphorylating perilipin (1,2,4,5). Phosphorylation of perilipin results in the conformational change that exposes lipid droplets to endogenous lipases, such as hormone-sensitive lipases (2). Hence, perilipin plays a pivotal role in lipid storage (2,5).

  1. Greenberg, A.S. et al. (1991) J Biol Chem 266, 11341-6.
  2. Brasaemle, D.L. (2007) J Lipid Res 48, 2547-59.
  3. Ducharme, N.A. and Bickel, P.E. (2008) Endocrinology 149, 942-9.
  4. Egan, J.J. et al. (1990) J Biol Chem 265, 18769-75.
  5. Brasaemle, D.L. et al. (2009) Mol Cell Biochem 326, 15-21.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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