Product Pathways - Cytoskeletal Signaling
Keratin 7 Antibody #3473
Have you tried your application using our XP® monoclonal antibodies? Try product: 4465
PhosphoSitePlus® protein, site, and accession data: K7
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (Mk) | Endogenous | 52 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3473:
- Western Blotting
Specificity / Sensitivity
Keratin 7 Antibody detects endogenous levels of keratin 7 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human keratin 7. Polyclonal antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analyses of extracts of various cell lines using Keratin 7 Antibody. Note that MCF7 are negative for Keratin 7, as expected.
Background
Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).
Keratin 7 and Keratin 19 are present in hepatic and pancreatic progenitor/stem cells (7,8).
- Moll, R. et al. (1982) Cell 31, 11-24.
- Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
- Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
- Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
- Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
- Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.
- Iyer, A. et al. (2008) Hum. Pathol. 39, 1370-1377.
- Meier, K. et al. (2009) Ann Anat 191, 70-82.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3984 Keratin 17/19 (D32D9) XP® Rabbit mAb
- 4548 Keratin 18 (DC10) Mouse mAb
- 4558 Keratin 19 (BA17) Mouse mAb
- 3479 Keratin 19 Antibody
- 4546 Keratin 8/18 (C51) Mouse mAb
- 4523 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 4528 Pan-Keratin (C11) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 4545 Pan-Keratin (C11) Mouse mAb
- 3519 Phospho-Keratin 17 (Ser44) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7076 Anti-mouse IgG, HRP-linked Antibody
For Research Use Only. Not For Use In Diagnostic Procedures.
