Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb #3474
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W | H M | 160 | Rabbit | IgG |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-FLT3 (Tyr591) (33G6) Rabbit mAb detects endogenous levels of FLT3 only when phosphorylated at tyrosine 591. The antibody may cross-react with some tyrosine-phosphorylated proteins.
Source / Purification
Monoclonal antibody is produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to the sequence surrounding Tyr591 of human FLT3.
Western Blotting
Western blot analysis of SEM cell lysate untreated or treated with FLT3 inhibitor, using Phospho-FLT3 (Tyr591) Rabbit mAb (upper) and FLT3 (8F2) Rabbit mAb #3462 (lower).
Background
FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).
- Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev. 9, 37-48.
- Naoe, T. et al. (2001) Cancer Chemother. Pharmacol. 48 Suppl1, S27-S30.
- Namikawa, R. et al. (1996) Stem Cells 14, 388-395.
- Beslu, N. et al. (1996) J. Biol. Chem. 271, 20075-20081.
- Zhang, S. and Broxmeyer, H.E. (2000) Biochem. Biophys. Res. Commun. 277, 195-199.
- Zhang, S. et al. (1999) J. Leukoc. Biol. 65, 372-380.
- Mizuki, M. et al. (2000) Blood 96, 3907-3914.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3461 Phospho-FLT3 (Tyr591) Antibody
- 3462 FLT3 (8F2) Rabbit mAb
- 3464 Phospho-FLT3 (Tyr589/591) (30D4) Rabbit mAb
- 3466 Phospho-FLT3 (Tyr591) (54H1) Mouse mAb
- 3459 Phospho-FLT3 (Tyr591) Antibody (Alexa Fluor® 488 Conjugate)
- 7743 HTScan® FLT3 Kinase Assay Kit
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3463 Phospho-FLT3 (Tyr969) (C24D9) Rabbit mAb
- 4577 Phospho-FLT3 (Tyr842) (10A8) Rabbit mAb
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
