Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

LATS1 (C66B5) Rabbit mAb #3477

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Mk Endogenous 140 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LATS1 (C66B5) Rabbit mAb detects endogenous levels of total LATS1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptide corresponding to amino acids surrounding Gly180 of human LATS1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using LATS1 (C66B5) Rabbit mAb.

Background

LATS1 (Large tumor suppressor 1) is a putative serine/threonine kinase that belongs to the NDR family (1). It is a tumor suppressor that plays a critical role in the maintenance of ploidy. LATS1 localizes to the centrosome and the mitotic spindle and controls G2/M transition by negatively regulating cdc2 kinase activity (2,3). LATS1 also plays a role in the G1 tetraploidy checkpoint via control of p53 expression (4).LATS1 affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1 (5). LATS1 also binds the phosphorylated form of zyxin, a regulator of actin filament assembly. This interaction promotes localization of zyxin to the mitotic spindle, suggesting a role for actin regulatory proteins during mitosis (6). Decreased expression is associated with breast tumor aggressiveness (7), promoter methylation, and loss of heterozygosity. Mutations perturbing LATS1 have been associated with human sarcomas and ovarian sarcomas (8,9). LATS1 knock out mice develop soft-tissue sarcomas, ovarian stromal cell tumor, and display a high sensitivity to carcinogenic treatments (10).Human LATS1 exists in a complex similar to the Drosophila Hippo/Salvador/Lats tumor suppressor network, a complex that regulates proliferation and apoptosis to control growth and shape of the fly. The corresponding human complex contains Hippo and Salvador homologs RASSF1A, WW45, and MST2 and may control mitotic exit (11).Many molecules have been discovered that are involved in the Hippo pathway, but mechanisms governing activation of these proteins and activation of the pathway in general are currently not clear. Phosphorylation of LATS1 and LATS2 is stimulated by association with Mob1, which is itself phosphorylated by Mst1/2 (12,13). Phosphorylation of LATS1/2 is required for activation of YAP, a key mediator of the Hippo signaling pathway (14).

  1. Tao, W. et al. (1999) Nat Genet 21, 177-81.
  2. Yang, X. et al. (2001) Oncogene 20, 6516-23.
  3. Xia, H. et al. (2002) Oncogene 21, 1233-41.
  4. Iida, S. et al. (2004) Oncogene 23, 5266-74.
  5. Yang, X. et al. (2004) Nat Cell Biol 6, 609-17.
  6. Hirota, T. et al. (2000) J Cell Biol 149, 1073-86.
  7. Morinaga, N. et al. (2000) Int J Oncol 17, 1125-9.
  8. Hansen, L.L. et al. (2002) Cancer Genet Cytogenet 139, 1-8.
  9. Hisaoka, M. et al. (2002) Lab Invest 82, 1427-35.
  10. St John, M.A. et al. (1999) Nat Genet 21, 182-6.
  11. Guo, C. et al. (2007) Curr Biol 17, 700-5.
  12. Hergovich, A. et al. (2006) Biochem Biophys Res Commun 345, 50-8.
  13. Hirabayashi, S. et al. (2008) Oncogene 27, 4281-92.
  14. Zhao, B. et al. (2010) J Cell Sci 123, 4001-6.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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