Product Pathways - Tyrosine Kinase / Adaptors
Pyk2 (5E2) Mouse mAb #3480
|W IP||H M||Endogenous||116||Mouse IgG2a|
Reactivity Key: H=Human M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Pyk2 (5E2) Mouse mAb detects endogenous levels of total Pyk2 protein. It does not cross-react with other related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST-Pyk2 fusion protein containing residues surrounding the carboxy-terminal of human Pyk2.
Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).
- Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
- Lev, S. et al. (1995) Nature 376, 737-745.
- Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
- Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
- Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
- Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
- Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.
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For Research Use Only. Not For Use In Diagnostic Procedures.