Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Pyk2 (5E2) Mouse mAb #3480

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 116 Mouse IgG2a

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Pyk2 (5E2) Mouse mAb detects endogenous levels of total Pyk2 protein. It does not cross-react with other related proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-Pyk2 fusion protein containing residues surrounding the carboxy-terminal of human Pyk2.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Pyk2 (5E2) Mouse mAb.

Background

Protein tyrosine kinase Pyk2, also called CAKβ, RAFTK and CADTK, is a nonreceptor tyrosine kinase structurally related to focal adhesion kinase (FAK) (1-4). Pyk2 is predominantly expressed in cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for the G-protein-coupled receptors and MAP kinase signaling pathway. It plays an important role in cell spreading and migration (5-7).

  1. Avraham, S. et al. (1995) J. Biol. Chem. 270, 27742-27751.
  2. Lev, S. et al. (1995) Nature 376, 737-745.
  3. Sasaki, H. et al. (1995) J. Biol. Chem. 270, 21206-21219.
  4. Yu, H. et al. (1996) J. Biol. Chem. 271, 29993-29998.
  5. Duong, L. T. et al. (2001) J. Biol. Chem. 276, 7484-7492.
  6. Watson, J. M. et al. (2001) J. Biol. Chem. 276, 3536-3542.
  7. Tang, H. et al. (2002) J. Biol. Chem. 277, 5441-5447.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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