Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Phospho-Ephrin B (Tyr324/329) Antibody #3481

Applications Reactivity Sensitivity MW (kDa) Source
W H Transfected Only 59 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Ephrin B (Tyr324/329) Antibody detects transfected levels of ephrin B protein only when phosphorylated at tyrosines 324/329. The antibody cross-reacts with ephrins B1, B2 and B3 but does not cross-react with other tyrosine-phosphorylated proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr324/329 of human ephrin B1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells stably expressing ephrin B1, stimulated with its cognate receptor EphB2-Fc or with unfused Fc, using Phospho-Ephrin B (Tyr324/329) Antibody (upper) or ephrin B antibody (lower).

Background

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands: EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand; EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5). Various stimuli can induce tyrosine phosphorylation of ephrin B, including binding to EphB receptors, activation of Src kinase, and stimulation by PDGF and FGF (6). Tyr324 and Tyr327 have been identified as major phosphorylation sites of ephrin B1 in vivo (7).

  1. Wilkinson, D.G. (2000) Int. Rev. Cytol. 196, 177-244.
  2. Klein, R. (2001) Curr. Opin. Cell Biol. 13, 196-203.
  3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-5619.
  4. Holder, N. and Klein, R. (1999) Development 126, 2033-2044.
  5. Brückner, K. et al. (1997) Science 275, 1640-1643.
  6. Palmer, A. et al. (2002) Mol. Cell 9, 725-737.
  7. Kalo, M.S. et al. (2001) J. Biol. Chem. 276, 38940-38948.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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