Cell Signaling Technology

Product Pathways - MAPK Signaling

MKP5 Antibody #3483

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 54 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MKP5 Antibody detects endogenous levels of total MKP5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human MKP5. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from RD and rat muscle cells using MKP5 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from C2C12 cells, differentiated for the indicated time periods, using MKP5 Antibody.

Background

MAP kinases are inactivated by dual-specificity protein phosphatases known as MAPK phosphatases (MKP), that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. MKPs specifically dephosphorylate both threonine and tyrosine residues in MAPK P-loops and have been shown to play important roles in regulating the function of the MAPK family (1,2). At least 13 members of the family (MKP-1, PAC-1, VHR, MKP-2, HVH3, MKP-3, PYST2, HVH5, MKP-4, MKP-5, MKP-6, MKP-7, DSP2) display unique substrate specificities for various MAP kinases (3). MAPK phosphatases typically contain an amino-terminal rhodanese-fold responsible for MKP docking to MAPK family members and a carboxy-terminal catalytic domain (4). These phosphatases can play important roles in development, immune system function, stress responses and metabolic homeostasis (5), and also in the development of cancer and the response of cancer cells to chemotherapy (6).

Dual specificity phosphatase 10 (DUSP10 or MKP5) selectively phosphorylates and inactivates p38α MAP kinase and JNK, but does not appear to affect p44/42 MAPK. Activated JNK phosphorylates the ATF2 transcription factor during periods of oxidative stress, which induces expression of MKP5 and related phosphatases. Increased MKP5 activity helps to further coordinate JNK activity during the stress response (7). Studies using MKP5 deficient mice demonstrated a likely role of this phosphatase in both the adaptive and innate immune responses (8).

  1. Camps, M. et al. (2000) FASEB J 14, 6-16.
  2. Theodosiou, A. and Ashworth, A. (2002) Genome Biol 3, REVIEWS3009.
  3. Salojin, K. and Oravecz, T. (2007) J Leukoc Biol 81, 860-9.
  4. Tanoue, T. et al. (2002) J Biol Chem 277, 22942-9.
  5. Dickinson, R.J. and Keyse, S.M. (2006) J Cell Sci 119, 4607-15.
  6. Wu, G.S. (2007) Cancer Metastasis Rev 26, 579-85.
  7. Teng, C.H. et al. (2007) J Biol Chem 282, 28395-407.
  8. Zhang, Y. et al. (2004) Nature 430, 793-7.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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