Cell Signaling Technology

Product Pathways - MAPK Signaling

HGK Antibody #3485

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 150 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

HGK Antibody detects endogenous levels of total HGK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human HGK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using HGK Antibody.

Background

HGK (MAP4K4 or mitogen-activated protein kinase kinase kinase kinase 4) is a serine/threonine kinase that belongs to the mammalian STE20/MAP4K kinase family involved in response to environmental stress and cytokines such as TNF-α (1-3). HGK specifically activates the c-Jun N-terminal kinase (JNK) signaling pathway and increases AP-1-mediated transcriptional activity in vivo (1). HGK is broadly expressed in many types of human cancer and cancer cell lines and plays an important role in cell transformation, invasiveness, adhesion and migration (4,5).

  1. Yao, Z. et al. (1999) J Biol Chem 274, 2118-25.
  2. Tesz, G.J. et al. (2007) J Biol Chem 282, 19302-12.
  3. Bouzakri, K. and Zierath, J.R. (2007) J Biol Chem 282, 7783-9.
  4. Wright, J.H. et al. (2003) Mol Cell Biol 23, 2068-82.
  5. Collins, C.S. et al. (2006) Proc Natl Acad Sci USA 103, 3775-80.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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