Cell Signaling Technology

Product Pathways - Neuroscience

KCNE1 Antibody #3486

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 15 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

KCNE1 Antibody detects endogenous levels of total KCNE1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp76 of human KCNE1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KCNE1 Antibody.

Background

Voltage-gated potassium channels play a variety of important roles in human health and disease (1,2). KCNE1, also known as MinK, belongs to a family of small transmembrane proteins (KCNE1, -2, -3, and -4 and KCNE1L) that modulate the activity of several voltage-gated K+ channels (3-5). KCNE1 functions as a modulatory β-subunit of the pore-forming α-subunit KCNQ1, and alters several biophysical properties of KCNQ1 channels (6,7). Several inherited mutations in KCNE1 result in long QT syndrome (8-10) and deafness (11).

  1. Jespersen, T. et al. (2005) Physiology (Bethesda) 20, 408-16.
  2. Robbins, J. (2001) Pharmacol Ther 90, 1-19.
  3. Takumi, T. et al. (1988) Science 242, 1042-5.
  4. Abbott, G.W. and Goldstein, S.A. (2001) Mol Interv 1, 95-107.
  5. McCrossan, Z.A. and Abbott, G.W. (2004) Neuropharmacology 47, 787-821.
  6. Barhanin, J. et al. (1996) Nature 384, 78-80.
  7. Sanguinetti, M.C. et al. (1996) Nature 384, 80-3.
  8. Splawski, I. et al. (1997) Nat Genet 17, 338-40.
  9. Abbott, G.W. and Goldstein, S.A. (2002) FASEB J 16, 390-400.
  10. Tian, C. et al. (2007) Biochemistry 46, 11459-72.
  11. Peters, T.A. et al. (2004) Pediatr Nephrol 19, 1194-201.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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