Cell Signaling Technology

Product Pathways - MAPK Signaling

MSK1 (C27B2) Rabbit mAb #3489

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 90 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MSK1 (C27B2) Rabbit mAb detects endogenous levels of total MSK1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn784 of human MSK1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MSK1 (C27B2) Rabbit mAb.

Background

MSK1, a mitogen and stress activated protein kinase, is activated by Erk as well as p38 MAPK in response to growth factors and cellular stress, respectively (1). MSK1 resembles RSK because it has two kinase domains connected by a regulatory linker region (2). Phosphorylation of RSK1 at Ser364 and Ser381 is critical for RSK1 activity (3). These sites are analogous to Ser360 and Ser376 of MSK1, which may be important for MSK1 activity as well.

  1. Deak, M. et al. (1998) EMBO J. 17, 4426-4441.
  2. Pierrat, B. et al. (1998) J. Biol. Chem. 273, 29661-29671.
  3. Dalby, K. et al. (1998) J. Biol. Chem. 273, 1496-1505.

Application References

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Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

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