Product Pathways - Cytoskeletal Signaling
Cortactin (H222) Antibody #3503
|3503S||100 µl (10 western blots)||---||In Stock||---|
|3503||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Monkey, Bovine||Endogenous||80-85||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
Cortactin (H222) Antibody detects endogenous levels of total cortactin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to centrally located residues of human cortactin. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from various cell types using Cortactin (H222) Antibody.
Immunoprecipitation of cortactin from HeLa cell lysates using Cortactin (H222) Antibody. Western blot was performed using the same antibody.
Confocal immunofluorescent analysis of HeLa cells using Cortactin (H222) Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Cortactin is a cortical actin binding protein. Its amino-terminal acidic domain (NTA) associates with the Arp2/3 and WASP complex at F-actin branches. The central region of the protein contains six repeats of 37 amino acids that are important in F-actin binding and cross-linking. The carboxy-terminus contains a proline-rich region and an SH3 domain that can interact with numerous scaffolding proteins, such as CortBP1 and Shank3 (1,2). Cortactin is involved in signaling events that coordinate actin reorganization during cell movement. The human cortactin homologue EMS1 is overexpressed in numerous cancers with poor patient prognosis (3). Cortactin may also play an important role in the organization of transmembrane receptors at postsynaptic densities (PSD) and tight junctions by linking scaffolding proteins to the actin network (4).
Cortactin is phosphorylated at tyrosine residues 421, 466, and 482. Tyrosine phosphorylation of cortactin regulates cell motility (5), rac1-mediated actin dynamics (6), cadherin-dependent adhesion (7), chemokine trafficking and chemokine-dependent chemotaxis (8).
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- Rodrigo, J.P. et al. (2000) Clin Cancer Res 6, 3177-82.
- Weed, S.A. and Parsons, J.T. (2001) Oncogene 20, 6418-34.
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- Head, J.A. et al. (2003) Mol Biol Cell 14, 3216-29.
- El Sayegh, T.Y. et al. (2004) J Cell Sci 117, 5117-31.
- Luo, C. et al. (2006) J Biol Chem 281, 30081-93.
- Eke, I. et al. (2012) J Clin Invest 122, 1529-40. Applications: Western Blotting.
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