Product Pathways - NF-kB Signaling
TBK1/NAK (D1B4) Rabbit mAb #3504
PhosphoSitePlus® protein, site, and accession data: TBK1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H M R Mk | Endogenous | 84 | Rabbit IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
TBK1/NAK (D1B4) Rabbit mAb detects endogenous levels of total TBK1/NAK protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser645 of human TBK1/NAK.
Western Blotting
Western blot analysis of extracts from various cell lines using TBK1/NAK (D1B4) Rabbit mAb.
Background
TBK1 (TANK-binding kinase 1)/NAK (NF-κB activating kinase) is an IκB kinase (IKK)-activating kinase and can activate IKK through direct phosphorylation (1). TBK1 was identified through association with the TRAF binding protein, TANK, and found to function upstream of NIK and IKK in the activation of NF-κB (2). TBK1/NAK induces IκB degradation and NF-κB activity through IKKβ. NAK may mediate IKK and NF-κB activation in response to growth factors that stimulate PKCε activity (1). TBK1 plays a pivotal role in the activation of IRF3 in the innate immune response (3).
- Tojima, Y. et al. (2000) Nature 404, 778-82.
- Pomerantz, J.L. and Baltimore, D. (1999) EMBO J 18, 6694-704.
- Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3013 TBK1/NAK Antibody
- 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
