Cell Signaling Technology

Product Pathways - Development

Sav1 Antibody #3507

Applications Reactivity Sensitivity MW (kDa) Source
W IP H R Mk Endogenous 45 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Sav1 Antibody detects endogenous level of total Sav1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala100 of human Sav1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Sav1 Antibody.

Background

Sav1 (Salvador homolog 1) was first identified as a 45 kDa protein containing a pair of WW domains and a coiled-coil region. Originally named WW45, Sav1 is ubiquitously expressed in adult tissues but is also present during specific developmental periods. Sav1 acts a scaffold protein in a complex that includes the kinases MST2 and LATS1, and a YAP transcriptional co-activator (3). This group of proteins functions in the newly identified Hippo pathway, which regulates important developmental functions such as cell contact inhibition and organ size. Further evidence suggests a role in tumor suppression and cancer pathogenesis (4,5). The Hippo pathway requires Sav1 for activation of MST1 and the resulting activation of LATS1/2 (6). Embryonic mice lacking Sav1 display hyperplastic growth and epithelial differentiation defects, implying that Sav1 plays a prominent role epithelial cell differentiation (7).

  1. Valverde, P. (2000) Biochem Biophys Res Commun 276, 990-8.
  2. Tapon, N. et al. (2002) Cell 110, 467-78.
  3. Oka, T. et al. (2008) J Biol Chem 283, 27534-46.
  4. Guo, C. et al. (2007) Curr Biol 17, 700-5.
  5. Zeng, Q. and Hong, W. (2008) Cancer Cell 13, 188-92.
  6. Edgar, B.A. (2006) Cell 124, 267-73.
  7. Lee, J.H. et al. (2008) EMBO J 27, 1231-42.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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