Product Pathways - Adhesion
Phospho-Connexin 43 (Ser368) Antibody #3511
|3511S||100 µl (10 western blots)||---||In Stock||---|
|3511||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Hamster, Monkey, Dog||Endogenous||42, 44, 46||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Species predicted to react based on 100% sequence homology: Pig.
Specificity / Sensitivity
Phospho-Connexin 43 (Ser368) Antibody detects endogenous levels of connexin 43 only when phosphorylated at serine 368. The antibody does not cross-react with other phosphorylated connexin family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser368 of human connexin 43. Antibodies are purified by protein A and peptide affinity chromatography.
Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5).
- Solan, J. L. et al. (2003) . J. Cell Sci. 116 (11), 2203-2211. Applications: Western Blotting, IC-IF, IP.
- Matsushita, S. et al. (2006) J Histochem Cytochem 54, 343-53. Applications: Western Blotting, IC-IF.
- Ek-Vitorin, J.F. et al. (2006) Circ Res 98, 1498-505. Applications: Western Blotting, IF-P (paraffin).
- Miura, T. et al. (2007) Am J Physiol Heart Circ Physiol 293, H1425-31. Applications: Western Blotting.
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