Cell Signaling Technology

Product Pathways - Adhesion

Phospho-Connexin 43 (Ser368) Antibody #3511

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Hm Endogenous 42, 44, 46 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Hm=Hamster
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Connexin 43 (Ser368) Antibody detects endogenous levels of connexin 43 only when phosphorylated at serine 368. The antibody does not cross-react with other phosphorylated connexin family members.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser368 of human connexin 43. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS and C6 cells, untreated or PMA-treated, using Phospho-Connexin 43 (Ser368) Antibody.

Background

Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5).

  1. Musil, L. S. et al. (1990) J. Cell. Biol. 111, 2077-2088.
  2. Musil, L.S. and Goodenough, D.A. (1991) J. Cell. Biol. 115, 1357-1374.
  3. Lampe, P. D. et al. (2000) J. Cell. Biol. 149, 1503-1512.
  4. Giepmans, B. N. et al. (2001) J. Biol. Chem. 276, 8544-8549.
  5. Lin, R. et al. (2001) J. Cell. Biol. 154, 815-827.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes