Cell Signaling Technology

Product Pathways - Adhesion

Connexin 43 Antibody #3512

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P IHC-F IF-F IF-IC H M R Mk Z (Dg) (Pg) Endogenous 39, 41, 43, 44 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IHC-F=Immunohistochemistry (Frozen)  IF-F=Immunofluorescence (Frozen)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Z=Zebrafish  Dg=Dog  Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Connexin 43 Antibody detects endogenous levels of total connexin 43. This antibody does not cross-react with other connexins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues of human connexin 43. Antibodies are purified by protein A and peptide affinity chromatography

Western Blotting

Western Blotting

Western blot analysis of extracts from untreated and PMA-treated C6, COS, HeLa and C2C12 cells using Connexin 43 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Connexin 43 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human heart, using Connexin 43 Antibody. (High magnification, inset).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Connexin 43 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse heart using Connexin 43 Antibody in the presence of control peptide (left) or antigen specific peptide (lright).

IHC-F (frozen)

IHC-F (frozen)

Immunohistochemical analysis of frozen mouse heart tissue, using Connexin 43 Antibody.


IF-IC

IF-IC

Confocal immunofluorescent analysis of confluent COS cells using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

IF-F

IF-F

Confocal immunofluorescent analysis of rat optic nerve and ciliary epithelium using Connexin 43 Antibody (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).See notes for additional testing details.

Background

Connexin 43 (Cx43) is a member of the large family of gap junction proteins. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions (1,2). Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication (3). Src can interact with and phosphorylate Cx43 to alter gap junction communication (4,5).

  1. Musil, L. S. et al. (1990) J. Cell. Biol. 111, 2077-2088.
  2. Musil, L.S. and Goodenough, D.A. (1991) J. Cell. Biol. 115, 1357-1374.
  3. Lampe, P. D. et al. (2000) J. Cell. Biol. 149, 1503-1512.
  4. Giepmans, B. N. et al. (2001) J. Biol. Chem. 276, 8544-8549.
  5. Lin, R. et al. (2001) J. Cell. Biol. 154, 815-827.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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