Product Pathways - Companion Products
GST (26H1) Mouse mAb (Sepharose Bead Conjugate) #3513
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IP | All | Transfected Only | Mouse IgG2a |
Applications Key:
IP=Immunoprecipitation
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
Protocols
- 3513:
- Immunoprecipitation
Specificity / Sensitivity
GST (26H1) Mouse mAb (Sepharose Bead Conjugate) detects transfected GST fusion proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein.
IP
Immunoprecipitation of extracts from COS cells transfected with a construct overexpressing TrCp1-GST using GST (26H1) Mouse mAb (Sepharose Bead Conjugate) (lane 2). Mouse IgG Isotype Control (Sepharose Bead Conjugate) #3420 (lane 1) was used as a negative control. The western blot was probed using GST (91G1) Rabbit mAb #2625.
Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. GST (26H1) Mouse mAb (Sepharose Bead Conjugate) is useful for immunoprecipitation assays.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable Tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
- Guan, K.L. and Dixon, J.E. (1991) Anal Biochem 192, 262-7.
- Davies, A.H. et al. (1993) Biotechnology (NY) 11, 933-6.
- Yu, J. et al. (1998) Mol Cell Biol 18, 1379-87.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2624 GST (26H1) Mouse mAb
- 2625 GST (91G1) Rabbit mAb
- 3400 Myc-Tag (9B11) Mouse mAb (Sepharose Bead Conjugate)
- 3420 Mouse IgG (Sepharose Bead Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
