Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

MLH1 (4C9C7) Mouse mAb #3515

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H Mk Endogenous 85 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MLH1 (4C9C7) Mouse mAb detects endogenous levels of total MLH1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with truncated recombinant MBP-MLH1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using MLH1 (4C9C7) Mouse mAb.

IP

IP

MLH1 was immunoprecipitated from HeLa cell lysates using MLH1 (4C9C7) Mouse mAb. Western blot was performed using the same antibody. Lane 1 is 5% input.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of IGROV-1 cells (red) and Jurkat cells (blue) using MLH1 (4C9C7) Mouse mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using MLH1 (4C9C7) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Background

Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity. MLH1 is the human homologue of the E. coli MMR gene mutL. MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-γ), PMS2 (MutL-α) or MLH3 (MutL-γ). Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1). Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5). The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6). MLH1 also plays a role in meiotic recombination (7).

  1. Modrich, P. (2006) J Biol Chem 281, 30305-9.
  2. Seng, T.J. et al. (2008) Br J Cancer 99, 375-82.
  3. Harley, I. et al. (2008) Gynecol Oncol 109, 384-7.
  4. Mao, G. et al. (2008) J Biol Chem 283, 3211-6.
  5. Hubner, R.A. and Houlston, R.S. (2007) J Natl Cancer Inst 99, 1490; author reply 1490-1.
  6. Vasen, H.F. (2005) Fam Cancer 4, 219-25.
  7. Argueso, J.L. et al. (2003) Mol Cell Biol 23, 873-86.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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