Product Pathways - Lymphocyte Signaling
CD44 (156-3C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #3516
| Applications | Reactivity | Source | Isotype |
|---|---|---|---|
| F | H | Mouse | IgG2a |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CD44 (156-3C11) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total CD44 protein.
Source / Purification
Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-5.
Flow Cytometry
Flow cytometric analysis of HeLa cells using CD44 (156-3C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells using CD44 (156-3C11) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells.
Background
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1-2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3).CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).
- Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
- Cichy, J. and Pure, E. (2003) J. Cell Biol. 161, 839-843.
- Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
- Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
- Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
- Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.
Application References
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