Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

GADD45 α Antibody #3518

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 22 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

GADD45 α Antibody detects endogenous levels of total GADD45 α protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 α. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa cells, untreated or treated with UV or doxirubicin, using GADD45 α Antibody.

Background

GADD45 α, β and γ are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents (1). GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase (2). GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators (3,4).

  1. Wang, X.W. et al. (1999) Proc Natl Acad Sci USA 96, 3706-11.
  2. Yang, Q. et al. (2000) J Biol Chem 275, 36892-8.
  3. Zhao, H. et al. (2000) Exp Cell Res 258, 92-100.
  4. Liebermann, D.A. and Hoffman, B. (2008) J Mol Signal 3, 15.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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