Product Pathways - Cell Cycle / Checkpoint
Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb #3520
PhosphoSitePlus® protein, site, and accession data: NPM1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W F | H (M) (R) (Mk) (X) (B) (Dg) | Endogenous | 38 | Rabbit IgG |
Applications Key:
W=Western Blotting
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
X=Xenopus
B=Bovine
Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3520:
- Flow, Western Blotting
Specificity / Sensitivity
Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb detects endogenous levels of NPM only when phosphorylated at Ser4.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser4 of human NPM. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts of HeLa cells, either untreated or nocodazole-treated, using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb (upper). Lower panel shows total NPM using NPM Antibody #3542.
Flow Cytometry
Flow cytometric analysis of Jurkat cells using Phospho-NPM (Ser4) (D19C1) XP® Rabbit mAb compared to Propidium Iodide (PI)/RNase Staining Solution #4087. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Background
Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase α activity, centrosome duplication and molecular chaperoning activities (1,2). The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase (3,4).
- Okuda, M. et al. (2000) Cell 103, 127-140.
- Takemura, M. et al. (1999) J. Biochem. (Tokyo) 125, 904-909.
- Morris, S.W. et al. (1994) Science 263, 1281-1284.
- Bischof, D. et al. (1997) Mol. Cell. Biol. 17, 2312-2325.
Application References
- So, C.H. et al. (2012) J Biol Chem 287, 17088-99. Applications: Western Blotting
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Companion Products
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
