Cell Signaling Technology

Product Pathways - DNA Damage

Phospho-MDM2 (Ser166) Antibody #3521

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 90 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-MDM2 (Ser166) Antibody detects endogenous levels of MDM2 only when phosphorylated at Ser166.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser166 of human MDM2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from COS cells, untransfected or transfected with Wild-type or mutant (S166S186A) MDM2, using Phospho-MDM2 (Ser166) Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from MCF-7 cells treated with IGF-1 for the indicated times, using Phospho-MDM2 (Ser166) Antibody.

Background

MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).

  1. Haupt, Y. et al. (1997) Nature 387, 296-299.
  2. Mayo, L.D. and Donner, D.B. (2001) Proc. Natl. Acad. Sci. USA 98, 11598-11603.
  3. Zhou, B. P. et al. (2001) Nat. Cell Biol. 3, 973-981.
  4. Grossman, S. R. et al. (1998) Mol. Cell 2, 405-415.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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