Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

Phospho-PNK1 (Ser114/Thr118) Antibody #3522

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 59 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-PNK1 (Ser114/Thr118) Antibody detects endogenous levels of PNK1 only when phosphorylated on Ser114 and Thr118.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser114 and Thr118 of human PNK1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts of 293 and HeLa cells, either untreated or treated with UV radiation, using Phospho-PNK1 (Ser114/Thr118) Antibody (upper). β-actin Antibody #4967 (lower) was used to control for loading.

Background

PNK (polynucleotide kinase) is a DNA repair enzyme that participates in single strand break repair and non-homologous end rejoining (NHEJ) for double strand breaks. PNK possesses a 5'-DNA kinase activity and a 3'-DNA phosphatase activity (1,2). It has three domains, a C-terminal kinase domain, a central phosphatase domain, and an N-terminal forkhead associated (FHA) domain that is responsible for protein-protein interactions. Reduction in expression of PNK by RNAi sensitizes cells to ionizing radiation and topoisomerase I inhibitors (3)

Phosphorylation of PNK1 at Ser114/ Thr118 was independently identified at Cell Signaling Technology (CST) using PhosphoScan®, CST's MS/MS platform for phosphorylation site discovery. Phosphorylation of PNK1 at Ser114/ Thr118 was observed in extracts of various cell lines following UV treatment. For additional information please visit PhosphoSitePlus®, CST's modification site knowledgebase, at www.phosphosite.org.

  1. Jilani, A. et al. (1999) J Biol Chem 274, 24176-86.
  2. Dobson, C.J. and Allinson, S.L. (2006) Nucleic Acids Res 34, 2230-7.
  3. Bernstein, N.K. et al. (2008) Anticancer Agents Med Chem 8, 358-67.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products