Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

RalB Antibody #3523

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 26 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RalB Antibody detects endogenous levels of total RalB protein. The antibody might also react with overexpressed RalA protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu142 of human RalB protein. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using RalB Antibody.

Background

RalA and RalB are members of the Ras family of small GTPases and are highly homologous in protein sequence. The functions of RalA and RalB are distinct yet overlapping. By binding to various effector proteins, RalA and RalB serve as important GTP sensors for exocytosis and membrane trafficking (1-3). RalA is required for Ras-related tumorigenesis (4) and RalB is important for tumor survival (5). In addition to tumor formation, Ral proteins also play a role in cancer cell migration and metastatic tumor invasion (6,7).

  1. Li, G. et al. (2007) J Neurosci 27, 190-202.
  2. van Dam, E.M. and Robinson, P.J. (2006) Int J Biochem Cell Biol 38, 1841-7.
  3. Camonis, J.H. and White, M.A. (2005) Trends Cell Biol 15, 327-32.
  4. Lim, K.H. et al. (2005) Cancer Cell 7, 533-45.
  5. Chien, Y. et al. (2006) Cell 127, 157-70.
  6. Oxford, G. et al. (2005) Cancer Res 65, 7111-20.
  7. Lim, K.H. et al. (2006) Curr Biol 16, 2385-94.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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