Product Pathways - Lymphocyte Signaling
CD31 (PECAM-1) (89C2) Mouse mAb #3528
PhosphoSitePlus® protein, site, and accession data: PECAM-1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H | Endogenous | 130 | Mouse IgG2b |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
CD31 (PECAM-1) (89C2) Mouse mAb detects endogenous levels of total CD31 protein. It does not cross-react with other related proteins.
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant human CD31 protein.
Western Blotting
Western blot analysis of extracts from HUVEC cells using CD31 (PECAM-1) (89C2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human smooth muscle using CD31 (PECAM-1) (89C2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HUVEC pellets using CD31 (PECAM-1) (89C2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human clear cell carcinoma using CD31 (PECAM-1) (89C2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human capillary hemangioma using CD31 (PECAM-1) (89C2) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells (red) and HUVEC (blue), using CD31 (PECAM-1) (89C2) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of HUVEC (left) and HeLa cells (right) using CD31 (PECAM-1) (89C2) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis (1). CD31 is phosphorylated on Tyr686 at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCγ and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
- Newman, P.J. (1997) J. Clin. Invest. 99, 3-8.
- Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-15772.
- Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-524.
Application References
- Whyte, J.L. et al. (2011) Stem Cell Res 6, 238-50. Applications: IC-IF Western Blotting
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Companion Products
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- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
