Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

SDF1 (D32F9) Rabbit mAb #3530

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H R (M) Recombinant 9 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SDF1 (D32F9) Rabbit mAb detects recombinant SDF1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human SDF1.

Western Blotting

Western Blotting

Western blot analysis of recombinant human SDF1 protein using SDF1 (D32F9) Rabbit mAb.

Background

The stromal cell derived factor 1 (SDF1, CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1 isoforms (SDF1α and SDF1β) that have different properties and biological activities (7). Additional isoforms of SDF1 have been reported (8,9).

  1. Maekawa, T. and Ishii, T. (2000) Intern. Med. 39, 90-100.
  2. Ray, N. and Doms, R.W. (2006) Curr. Top. Microbiol. Immunol. 303, 97-120.
  3. Sugiyama, T. et al. (2006) Immunity 25, 977-988.
  4. Ara, T. et al. (2003) Immunity 19, 257-267.
  5. Kucia, M. et al. (2004) J. Mol. Histol. 35, 233-245.
  6. Ratajczak, M.Z. et al. (2006) Leukemia 20, 1915-1924.
  7. De La Luz Sierra, M. et al. (2004) Blood 103, 2452-2459.
  8. Gleichmann, M. et al. (2000) Eur. J. Neurosci. 12, 1857-1866.
  9. Yu, L. et al. (2006) Gene 374, 174-179.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products