Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Btk Antibody #3532

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 77 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Btk Antibody detects endogenous levels of Btk. This antibody does not cross-react with other unrelated proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Tyr223 of human Btk. Antibodies are purified by protein A and peptide-affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of various cell lysates using BTK Antibody.

Background

Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity (8).

  1. Khan, W.N. (2001) Immunol. Res. 23, 147-156.
  2. Lewis, C.M. et al. (2001) Curr. Opin. Immunol. 13, 317-325.
  3. Salim, K. et al. (1996) EMBO J. 15, 6241-6250.
  4. Rameh, L.E. et al. (1997) J. Biol. Chem. 272, 22059-22066.
  5. Varnai, P. et al. (1999) J. Biol. Chem. 274, 10983-10989.
  6. Rawlings, D.J. et al. (1996) Science 271, 822-825.
  7. Park, H. et al. (1996) Immunity 4, 515-525.
  8. Kang, S.W. et al. (2001) EMBO J. 20, 5692-5702.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Products