Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Btk (C82B8) Rabbit mAb #3533

Applications Reactivity Sensitivity MW (kDa) Isotype
W H Endogenous 77 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Btk (C82B8) Rabbit mAb detects endogenous levels of total Btk. This antibody does not cross-react with other unrelated proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro80 of human Btk.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Btk (C82B8) Rabbit mAb.

Background

Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain, and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development (1,2). Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate (3-5). The membrane-located Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation (6,7). The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation and subsequent activation (8). The PKC inhibitory signal is likely to be a key determinant of the B-cell receptor signaling threshold to maintain optimal Btk activity (8).

  1. Khan, W.N. (2001) Immunol. Res. 23, 147-156.
  2. Lewis, C.M. et al. (2001) Curr. Opin. Immunol. 13, 317-325.
  3. Salim, K. et al. (1996) EMBO J. 15, 6241-6250.
  4. Rameh, L.E. et al. (1997) J. Biol. Chem. 272, 22059-22066.
  5. Varnai, P. et al. (1999) J. Biol. Chem. 274, 10983-10989.
  6. Rawlings, D.J. et al. (1996) Science 271, 822-825.
  7. Park, H. et al. (1996) Immunity 4, 515-525.
  8. Kang, S.W. et al. (2001) EMBO J. 20, 5692-5702.

Application References

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This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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