Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Lymphocyte Signaling

GATA-1 (D52H6) XP® Rabbit mAb #3535

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC F H M R Endogenous 43 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GATA-1 (D52H6) XP® Rabbit mAb detects endogenous levels of GATA-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu13 of human GATA-1.

Western Blotting

Western Blotting

Western blot analysis of lysates from various cell lines using GATA-1 (D52H6) XP® Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of formalin-fixed paraffin-embedded undecalcified mouse bone using GATA-1 (D52H6) XP® Rabbit mAb. Note staining of cells in the marrow.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells (red) and K-562 cells (blue) using GATA-1 (D52H6) XP® Rabbit mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of K-562 (left) and HeLa (right) cells using GATA-1 (D52H6) XP® Rabbit mAb (green) and S6 Ribosomal Protein (54D2) Mouse mAb #2317 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

GATA proteins comprise a group of transcription factors that are related by the presence of conserved zinc finger DNA binding domains, which bind directly to the nucleotide sequence core element GATA (1-3). There are six vertebrate GATA proteins, designated GATA-1 to GATA-6 (3).

GATA-1 is the founding member of the GATA family and is required for erythroid and megakaryocytic cell development (4,5). Mutations in the corresponding GATA-1 gene are linked to many human diseases, including acute megakaryoblastic leukemia in Down Syndrome children (DS-AMKL), X-linked thrombocytopenia, and gray platelet syndrome (6-9).

  1. Ko, L.J. and Engel, J.D. (1993) Mol Cell Biol 13, 4011-22.
  2. Merika, M. and Orkin, S.H. (1993) Mol Cell Biol 13, 3999-4010.
  3. Lowry, J.A. and Atchley, W.R. (2000) J Mol Evol 50, 103-15.
  4. Pevny, L. et al. (1991) Nature 349, 257-60.
  5. Fujiwara, Y. et al. (1996) Proc Natl Acad Sci USA 93, 12355-8.
  6. Wechsler, J. et al. (2002) Nat Genet 32, 148-52.
  7. Cantor, A.B. (2005) Int J Hematol 81, 378-84.
  8. Mehaffey, M.G. et al. (2001) Blood 98, 2681-8.
  9. Tubman, V.N. et al. (2007) Blood 109, 3297-9.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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