Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Rab11 Antibody #3539

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 25 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Rab11 Antibody detects endogenous levels of total Rab11 protein, including isoforms Rab11a and Rab11b.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg30 of human Rab11 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A549, Neuro-2A and COS cells using Rab11 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Rab11 Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Background

Rab11a, Rab11b and Rab25 are members of the Rab11 family of small Ras-like GTPases. Rab11 (isoforms Rab11a and Rab11b) functions as a key regulator in the recycling of perinuclear, plasma membrane and Golgi compartment endosomes (1,2). Despite some overlap, distinct differences exist between Rab11a and Rab11b in both their cellular distribution and functional roles. Rab11a is ubiquitously expressed while Rab11b is found mainly in the heart and brain (3,4). Like other Rab proteins, Rab11 exerts its function via interactions with Rab11 family interacting proteins (FIPs). While there are three distinct classes of FIPs, all appear to share a conserved carboxy-terminal Rab-binding domain that allows Rab-FIP protein interaction. When bound together, these proteins are thought to regulate membrane-associated protein sorting (5,6).

  1. Ullrich, O. et al. (1996) J. Cell Biol. 135, 913-924.
  2. Chen, W. et al. (1998) Mol. Biol. Cell 9, 3241-3257.
  3. Lapierre, L.A. et al. (2003) Exp. Cell Res. 290, 322-331.
  4. Khvotchev, M.V. et al. (2003) J. Neurosci. 23, 10531-10539.
  5. Junutula, J.R. et al. (2004) J. Biol. Chem. 279, 33430-33437.
  6. Hales, C.M. et al. (2001) J. Biol. Chem. 276, 39067-39075.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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