Product Pathways - Apoptosis
Bit1 Antibody #3543
PhosphoSitePlus® protein, site, and accession data: PTRH2
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 19, 21 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3543:
- Western Blotting
Specificity / Sensitivity
Bit1 Antibody detects endogenous levels of total human Bit1 protein. It also detects overexpressed bovine Bit1 and may detect overexpressed levels of mouse and rat Bit1.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues within a central region of Bit1. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa cells mock transfected or transfected with bovine Bit1, using Bit1 Antibody.
Western Blotting
Western blot analysis of extracts from K562, Raji, MCF7 and A204 cell lines, using Bit1 Antibody.
Background
Bit1 is a mitochondrial protein discovered as a regulator of anoikis, a process of apoptosis triggered by the loss of cell adhesion to the extracellular matrix (1,2). Like several other mitochondrial apoptotic regulators (Cytochrome c, Smac, HtrA2, AIF, EndoG), Bit1 is released into the cytoplasm during apoptosis (1,3). Cytoplasmic Bit1 associates with the Groucho family of transcriptional regulator AES (1,4). Interaction between Bit1 and AES promotes apoptosis, and this complex is suppressed by integrin mediated cell attachment.
- Jan, Y. et al. (2004) Cell 116, 751-762.
- Frisch, S.M. and Screaton, R.A. (2001) Curr. Opin. Cell Biol. 13, 555-562.
- Ekert, P.G. and Vaux, D.L. (2005) Curr. Opin. Cell Biol. 17, 626-630.
- Chen, G. and Courey, A.J. (2000) Gene 249, 1-16.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
