Cell Signaling Technology

Product Pathways - MAPK Signaling

Erk5 (D23E9) Rabbit mAb #3552

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 115 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Erk5 (D23E9) Rabbit mAb detects endogenous levels of total Erk5 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues in human Erk5.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Erk5 (D23E9) Rabbit mAb.

Background

ERK5 (Mitogen-activated protein kinase 7, Big mitogen-activated protein kinase 1) is a member of the MAPK superfamily implicated in the regulation numerous cellular processes including proliferation, differentiation, and survival (1,5-7). Like other MAPK family members, ERK5 contains a canonical activation loop TEY motif (Thr218/Tyr220) which is specifically phosphorylated by MAP2K5 (MEK5) in a growth factor-dependent, Ras-independent mechanism (2-4). For example, EGF stimulation promotes ERK5 phosphorylation which induces its traslocation to the nucleus where it phosphorylates MEF2C and other transcriptional targets (2,3). ERK5 is also activated in response to granulocyte colony-stimulating factor (G-CSF) in hematopoetic progenitor cells where it promotes survival and proliferation (4). In neuronal cells, ERK5 is required for NGF-induced neurite outgrowth, neuronal homeostatis, and survival (11,12). ERK5 is thought to play a role in blood vessel integrity via maintainence of endothelial cell migration and barrier function (8-10). Although broadly expressed, research studies have shown that mice lacking erk5 display numerous cardiac defects, suggesting ERK5 plays a critical role in vascular development and homeostasis (1,5).

  1. Zhou, G. et al. (1995) J Biol Chem 270, 12665-9.
  2. Kato, Y. et al. (1998) Nature 395, 713-6.
  3. Kato, Y. et al. (1997) EMBO J 16, 7054-66.
  4. Dong, F. et al. (2001) J Biol Chem 276, 10811-6.
  5. Hayashi, M. and Lee, J.D. (2004) J Mol Med 82, 800-8.
  6. Wang, X. and Tournier, C. (2006) Cell Signal 18, 753-60.
  7. Nishimoto, S. and Nishida, E. (2006) EMBO Rep 7, 782-6.
  8. Spiering, D. et al. (2009) J Biol Chem , .
  9. Sawhney, R.S. et al. (2009) J Cell Physiol 219, 152-61.
  10. Zhao, Z. et al. (2009) Mol Cell Biochem 322, 171-8.
  11. Obara, Y. et al. (2009) J Biol Chem , .
  12. Finegan, K.G. et al. (2009) Cell Death Differ 16, 674-83.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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