Product Pathways - Adhesion
Zyxin Antibody #3553
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M Mk | Endogenous | 82 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Protocols
- 3553:
- Western Blotting
Specificity / Sensitivity
Zyxin Antibody recognizes endogenous levels of total zyxin protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues in the central region of human zyxin. Antibodies are purified using protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using Zyxin Antibody.
Background
The zyxin family of proteins includes LIMD1, ajuba, trip6 and zyxin, each of which contains three LIM domains at the carboxy terminus. Zyxin family members associate with the actin cytoskeleton and are components of both the cell-cell junction adhesive complex and the integrin-mediated adhesive complex. They shuttle in and out of the nucleus where they may function in transcriptional activation (1). Zyxin is involved in the regulation of mechanical force-induced actin polymerization at focal adhesions (2), and in the regulation of adhesion and migration, possibly through the recruitment of Ena/VASP proteins to focal adhesions (3). Zyxin interacts with and may regulate the function of the tumor suppressor myopodin, which inhibits tumor growth and metastasis (4). Zyxin may be phosphorylated by Akt at Ser142 (5).
- Wang, Y. and Gilmore, T.D. (2003) Biochim Biophys Acta 1593, 115-20.
- Hirata, H. et al. (2008) J Cell Sci 121, 2795-804.
- Hoffman, L.M. et al. (2006) J Cell Biol 172, 771-82.
- Yu, Y.P. and Luo, J.H. (2006) Cancer Res 66, 7414-9.
- Chan, C.B. et al. (2007) Cell Death Differ 14, 1688-99.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4897 Ajuba Antibody
- 3389 LPP (8B3A11) Mouse mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
