Cell Signaling Technology

Product Pathways - Adhesion

Zyxin Antibody #3553

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Endogenous 78 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Zyxin Antibody recognizes endogenous levels of total zyxin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues in the central region of human zyxin. Antibodies are purified using protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Zyxin Antibody.

Background

The Ras superfamily of small GTP-binding proteins (G proteins) comprise a large class of proteins (over 150 members) that can be classified into at least five families based on their sequence and functional similarities: Ras, Rho, Rab, Arf, and Ran (1,2,3). All these small G proteins have both GDP/GTP-binding and GTPase activities and function as binary switches in diverse cellular and developmental events that include cell cycle preogression, cell survival, actin cytoskeletal organization, cell polarity and movement, and vesicular and nuclear transport (1). An upstream signal stimulates the dissociation of GDP from the GDP-bound form (inactive), which leads to the binding of GTP and entering the GTP-bound form (active). The activated GTP-bound form then goes through conformational change in the its downstream effector-binding region, eventually leads to binding and function regulation of downstream effectors. This activation can be switched off by the intrinsic GTPase activity, which hydrolyzes GTP to GDP and release the downstream effectors. These intrinsic guanine nucleotide exchange and GTP hydrolysis activities of Ras superfamily proteins are also regulated by guanine nucleotide exchange factors (GEFs) that promote formation of the active GTP-bound form and GTPase activating proteins (GAPs) that return the GTPase to its GDP-bound inactive form (4).

  1. Takai, Y. et al. (2001) Physiol Rev 81, 153-208.
  2. Colicelli, J. (2004) Sci STKE 2004, RE13.
  3. Wennerberg, K. et al. (2005) J Cell Sci 118, 843-6.
  4. Vigil, D. et al. (2010) Nat Rev Cancer 10, 842-57.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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