Product Pathways - Tyrosine Kinase/ Adaptors
Phospho-c-Cbl (Tyr774) Antibody #3555
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 120 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-c-Cbl (Tyr774) Antibody detects endogenous levels of c-Cbl only when phosphorylated at tyrosine 774. The antibody does not cross-react with related tyrosine-phosphorylated proteins.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Tyr774 of human c-Cbl. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from 293-T cells transfected with c-Cbl and Fyn (lanes 3 and 5) or without Fyn (lanes 2 and 4), untreated or calf intestinal phosphatase (CIP)-treated, using Phospho-c-Cbl (Tyr774) Antibody. (Lysates provided by Dr. A.L. Reddi, laboratory of Dr. Hamid Band, Harvard University, Massachusetts.)
Background
The c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells (1,2). c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol-3 kinase, Crk and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. Its RING finger domain recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for c-Cbl's ubiquitin ligase activity and downregulation of RTKs (3). In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5). Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn (6).
- Blake, T.J. et al. (1991) Oncogene 6, 653-657.
- Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.
- Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294-307.
- Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323-8331.
- Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.
- Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 3554 Phospho-c-Cbl (Tyr731) Antibody
- 2747 c-Cbl Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
