Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb #3556

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk Endogenous 90 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb detects endogenous levels of RSK2 only when phosphorylated at Ser227. It shows cross-reactivity with RSK1 when phosphorylated at the homologous serine residues.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser227 of human RSK2.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, COS and NIH/3T3 cells, treated with lambda-phosphatase or TPA/UV, using Phospho-RSK2 (Ser227) (D53A11) Rabbit mAb.

Background

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases characterized by two nonidentical, functional kinase domains (1) and a carboxy-terminal docking site for extracellular signal-regulated kinases (ERKs) (2). Several sites both within and outside of the RSK kinase domain, including Ser380, Thr359, Ser363, and Thr573, are important for kinase activation (3). RSK1-3 are activated via coordinated phosphorylation by MAPKs, by autophosphorylation, and by phosphoinositide-3-OH kinase (PI3K) in response to many growth factors, polypeptide hormones, and neurotransmitters (3).

PDK1 phosphorylates Ser227 in the activation loop of the amino-terminal kinase domain of RSK2, leading to substantial activation of the kinase in vitro and in vivo. The constitutively active PDK1 cooperates with ERK in the activation of RSK following the exposure of cells to growth factors or UV-light (4, 5).

  1. Fisher, T.L. and Blenis, J. (1996) Mol Cell Biol 16, 1212-9.
  2. Smith, J.A. et al. (1999) J Biol Chem 274, 2893-8.
  3. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505.
  4. Jensen, C.J. et al. (1999) J Biol Chem 274, 27168-76.
  5. Mérienne, K. et al. (2000) Oncogene 19, 4221-9.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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