Cell Signaling Technology

Product Pathways - Metabolism

LDHA/LDHC (C28H7) Rabbit mAb #3558

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P H M R Mk Endogenous 37 Rabbit IgG

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

LDHA/LDHC (C28H7) Rabbit mAb detects endogenous levels of total LDHA and LDHC proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using LDHA/LDHC (C28H7) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LDHA/LDHC (C28H7) Rabbit mAb.

Background

Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.

While LDHA is found primarily in muscle and kidney, LDHC is a testis-specific isoform (3). Both proteins are associated with human disease. Mutations in the corresponding LDHA gene are associated with LDHA deficiency, which is characterized by muscle stiffness following exercise and uterine stiffness during pregnancy (4). Abnormal LDHC expression is associated with several forms of cancer (5).

  1. Semenza, G.L. et al. (1996) J Biol Chem 271, 32529-37.
  2. Semenza, G.L. (2007) Biochem J 405, 1-9.
  3. Takano, T. and Li, S.S. (1989) Biochem Biophys Res Commun 159, 579-83.
  4. Miyajima, H. et al. (1993) Neurology 43, 1414-9.
  5. Koslowski, M. et al. (2002) Cancer Res 62, 6750-5.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.

For Research Use Only. Not For Use In Diagnostic Procedures.

Products