Cell Signaling Technology

Product Pathways - Protein Folding

CCT2 Antibody #3561

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 54 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CCT2 Antibody detects endogenous levels of total CCT2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human CCT2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CCT2 Antibody.

Background

CCT2 is one of eight largely unrelated subunit proteins found in a protein chaperone complex known as the chaperonin-containing TCP-1 (CCT) or TRiC complex. The CCT complex is an abundanct cytoslic component that is credited with helping newly synthesized polypeptides adopt the correct conformation (1). Proteins that fold and assemble with the help of CCT include the cytoskeletal proteins actin and tubulin as well as up to 15% of newly synthesized eukaryotic proteins (2). CCT2 is the β-subunit of the chaperone complex and is one of several CCT proteins that exhibit increased expression in response to stress. This implies that the CCT complex helps cells recover from protein damage by assisting in protein folding and assembly (3). CCT subunit levels also change throughout the cell cycle, with lower proteins levels (and reduced chaperone activity) found during induced cell cycle arrest during at M phase (4). Each CCT subunit is thought to perform a specific function during protein folding and assembly (5); CCT2 exhibits both actin and tubulin binding activities (6,3) but the exact molecular function on this subunit remains uncertain.

  1. Kubota, H. et al. (1995) Eur J Biochem 230, 3-16.
  2. Valpuesta, J.M. et al. (2002) FEBS Lett 529, 11-6.
  3. Yokota, S.I. et al. (2000) Eur J Biochem 267, 1658-64.
  4. Yokota, S. et al. (2001) Eur J Biochem 268, 4664-73.
  5. Kubota, H. et al. (1994) Curr Biol 4, 89-99.
  6. McCormack, E.A. et al. (2001) J Struct Biol 135, 198-204.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products