Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD4 (Edu-2) Mouse mAb #3563

Applications Reactivity Sensitivity MW (kDa) Isotype
F H Endogenous 55 Mouse IgG2a

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD4 (Edu-2) Mouse mAb detects endogenous levels of total CD4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of peripheral blood using CD4 (Edu-2) Mouse mAb.

Background

Cluster of Differentiation 4 (CD4) is a glycoprotein composed of an amino-terminal extracellular domain (four domains: D1-D4 with Ig-like structures), a transmembrane part and a short cytoplasmic tail. CD4 is expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages and dendritic cells, and plays an important role in the development and activation of T cells. On T cells, CD4 is the co-receptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the D1 domain of CD4 interacts with the β2-domain of the MHC class II molecule. CD4 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell and recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

  1. Zamoyska, R. (1994) Immunity 1, 243-246.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products