Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD10 (CB-CALLA) Mouse mAb #3565

Applications Reactivity Sensitivity MW (kDa) Isotype
F H Endogenous 100 Mouse IgG1

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

CD10 (CB-CALLA) Mouse mAb detects endogenous levels of CD10.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with CALLA cells.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of whole blood, using CD10 (CB-CALLA) Mouse mAb.

Background

CD10 (CALLA or neprilysin) was identified as a marker for leukemic cells in children with acute lymphoblastic leukemia and belongs to a family of exoenzymes that include aminopeptidase A, N and dipeptidylpeptidase IV (1-2). CD10 is a transmembrane type II molecule and functions as a metallo-peptidase requiring zinc. Specifically, CD10 cleaves 1-3 amino-terminal amino acids from peptides with a preference for neutral amino acids (valine, iso-leucine, phenylalanine, leucine or alanine) (2). The CD10 antigen is expressed by immature and by germinal center B cells, by granulocytes and by acute B-cell lymphoblastic leukemia cells. Therefore, CD10 appears to be involved in late stage regulation of hematopoietic cells (3).

  1. Greaves , M. F. et al. (1975) Clin. Immunol. Immunopathol. 4, 67-84.
  2. Shipp, M.A. and Look, A.T. (1993) Blood 82, 1052-1070.
  3. Bene, M. C. et al. (1997) Haematologica. 82, 205-210.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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