Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD10 (CB-CALLA) Mouse mAb #3565

Applications Reactivity Sensitivity MW (kDa) Isotype
F H Endogenous 100 Mouse IgG1

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD10 (CB-CALLA) Mouse mAb detects endogenous levels of CD10.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with CALLA cells.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of whole blood, using CD10 (CB-CALLA) Mouse mAb.

Background

CD10 (CALLA or neprilysin) was identified as a marker for leukemic cells in children with acute lymphoblastic leukemia and belongs to a family of exoenzymes that include aminopeptidase A, N and dipeptidylpeptidase IV (1-2). CD10 is a transmembrane type II molecule and functions as a metallo-peptidase requiring zinc. Specifically, CD10 cleaves 1-3 amino-terminal amino acids from peptides with a preference for neutral amino acids (valine, iso-leucine, phenylalanine, leucine or alanine) (2). The CD10 antigen is expressed by immature and by germinal center B cells, by granulocytes and by acute B-cell lymphoblastic leukemia cells. Therefore, CD10 appears to be involved in late stage regulation of hematopoietic cells (3).

  1. Greaves , M. F. et al. (1975) Clin. Immunol. Immunopathol. 4, 67-84.
  2. Shipp, M.A. and Look, A.T. (1993) Blood 82, 1052-1070.
  3. Bene, M. C. et al. (1997) Haematologica. 82, 205-210.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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