Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD16 (FcgammaIII) (CB-16) Mouse mAb #3567

Applications Reactivity MW (kDa) Source Isotype
F H 50 to 65 Mouse IgG1

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

CD16 (FcgammaIII) (CB-16) Mouse mAb detects endogenous levels of CD16.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of whole blood using CD16 (CB-16) Mouse mAb.

Background

CD64 (FcgammaRI), CD32 (FcgammaRII) and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32 and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).

  1. Maenaka, K. et al. (2001) J. Biol. Chem. 276, 44898-44904.
  2. Fridman, W. H. et al. (1992) Immunol. Rev. 125, 49-76.
  3. Pignata, C. et al. (1993) J. Immunol. 151, 6794-6800.

Application References

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