Product Pathways - Lymphocyte Signaling
CD31 (PECAM-1) (158-2B3) Mouse mAb #3568
| Applications | Reactivity | Sensitivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|---|
| F | H | Endogenous | 130 | Mouse | IgG1 |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CD31 (PECAM-1) (158-2B3) Mouse mAb detects endogenous levels of CD31.
Source / Purification
Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leucocytes.
Flow Cytometry
Flow cytometric analysis of whole blood, using CD31 (158-2B3) Mouse mAb.
Flow Cytometry
Two color flow cytometric analysis of whole blood, using CD31 (1582B3) Mouse mAb and CD5. Pink=granulocytes, blue=monocytes, and green=T cells & B cell subset.
Background
CD31 (Platelet Endothelial Cell Adhesion Molecule-1: PECAM-1), a member of the Ig superfamily of cell adhesion molecules, is expressed by circulating platelets, monocytes, neutrophils, some T cells and endothelial cells, and modulates cell adhesion, endothelial cell migration and angiogenesis (1). CD31 is tyrosine phosphorylated (Tyr686) at the cytoplasmic carboxy-terminal tail upon various stimuli (e.g. mechanical stress or oxidative stress), presumably by Src family members (2). The tyrosine phosphorylation mediates associations with a number of SH2 domain containing binding partners such as PI3-kinase, SHIP, PLCgamma and SHP-2. Thus, CD31 serves as a scaffold for various signaling molecules (3).
- Newman, P.J. (1997) J. Clin. Invest. 99, 3-8.
- Cao, M. Y. et al. (1998) J. Biol. Chem. 273, 15765-15772.
- Ilan, N. and Madri, J.A. (2003) Curr. Opin. Cell Biol. 15, 515-524.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
