Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD34 (ICO115) Mouse mAb #3569

Applications Reactivity MW (kDa) Source Isotype
IHC-P F H 110 Mouse IgG1

Applications Key:  IHC-P=Immunohistochemistry (Paraffin)  F=Flow Cytometry
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

CD34 (ICO115) Mouse mAb detects endogenous levels of total CD34 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with blast cells of a chronic myeloid leukemia patient.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human capillary hemangioma, using CD34 (ICO115) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of whole blood spiked with CD34-positive Stem-Trol™ Control Cells (Beckman Coulter) using CD34 (ICO-115) Mouse mAb.

Background

CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascular endothelium and some fibroblasts (1). CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimulation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention (2). The extracellular domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regulator of cell adhesion (3). CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34 (1,4).

  1. Felschow, D. M. et al. (2001) Blood 97, 3768-3775.
  2. Sato, T. et al. (1999) Blood 94, 2548-2554.
  3. Drew, E. et al. (2005) Immunity 22, 43-57.
  4. Fackler, M. J. et al. (1992) J. Biol. Chem. 267, 17540-17546.

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