Product Pathways - Lymphocyte Signaling
CD8 (RIV11) Mouse mAb #3572
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| F | H | Endogenous | 32 | Mouse IgG1 |
Applications Key:
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
CD8 (RIV11) Mouse mAb detects endogenous levels of total CD8 protein.
Source / Purification
Monoclonal antibody is produced by immunizing BALB/c mice with human peripheral lymphocytes.
Flow Cytometry
Flow cytometric analysis of whole blood, using CD8 (RIV11) Mouse mAb.
Background
Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen?Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8 α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR?antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 4443 CD3ε (CD3-12) Rat mAb
- 3563 CD4 (Edu-2) Mouse mAb
- 3565 CD10 (CB-CALLA) Mouse mAb
- 3566 CD13 (B-F10) Mouse mAb
- 3567 CD16 (FcgammaIII) (CB-16) Mouse mAb
- 3569 CD34 (ICO115) Mouse mAb
- 3576 CD56 (NCAM) (123C3) Mouse mAb
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
