Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD8 (RIV11) Mouse mAb #3572

Applications Reactivity Sensitivity MW (kDa) Isotype
F H Endogenous 32 Mouse IgG1

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD8 (RIV11) Mouse mAb detects endogenous levels of total CD8 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with human peripheral lymphocytes.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of peripheral blood using CD8 (RIV11) Mouse mAb.

Background

Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8 α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).

  1. Zamoyska, R. (1994) Immunity 1, 243-246.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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