Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD19 Antibody #3574

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC F H M Endogenous 95 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD19 Antibody detects endogenous levels of CD19 independent of its phosphorylated state.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around the carboxy-terminus of human CD19. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of SDS extracts from untreated or anti-human IgM treated (12 µg/ml for 2 minutes) Ramos cells and untreated or anti-mouse IgG treated (30 µg/ml for 2 minutes) A20 cells, using control CD19 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat T cells (blue) and Ramos B cells (green), using CD19 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Ramos (+) (left) and Jurkat (-) (right) cells using CD19 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

CD19 is a 95 kDa coreceptor, which amplifies the signaling cascade in B cells (1). On the B cell surface, CD19 associates with CD21, CD81 and Leu-13 to exert its function. The cytoplasmic tail of CD19 has nine conserved tyrosine residues playing critical roles in CD19 mediated function by coupling signaling molecules to the receptor (1). After B cell receptor or CD19 ligation, Tyr531 and Tyr500 of CD19 are progressively phosphorylated. This phosphorylation enables the coupling of PI3 kinase and Src family tyrosine kinase to CD19 and activates the PI3K and Src signaling pathways (2,3). Coligation of B cell receptor and CD19 also promotes Tyr409 phosphorylation in CD19. The phosphorylation at these sites enables its binding to Vav and mediates elevated intracellular calcium response, as well as the JNK pathway (4,5).

  1. Tedder, T.F. et al. (1997) Immunity 6, 107-18.
  2. Buhl, A.M. and Cambier, J.C. (1999) J Immunol 162, 4438-46.
  3. Fujimoto, M. et al. (2000) Immunity 13, 47-57.
  4. O'Rourke, L.M. et al. (1998) Immunity 8, 635-45.
  5. Sato, S. et al. (1997) Proc Natl Acad Sci U S A 94, 13158-62.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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