Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD56 (NCAM) (123C3) Mouse mAb #3576

Applications Reactivity Sensitivity MW (kDa) Isotype
W IHC-P IF-IC F H Endogenous 120 to 220 Mouse IgG1

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CD56 (NCAM) (123C3) Mouse mAb detects endogenous levels of total CD56 protein.

Source / Purification

Monoclonal antibody is produced by immunizing BALB/c mice with a membrane preparation of a small cell lung carcinoma specimen.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum using CD56 (NCAM) (123C3) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human neuroblastoma, using CD56 (NCAM) (123C3) Mouse mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of whole blood using CD56 (NCAM) (123C3) Mouse mAb.


IF-IC

IF-IC

Confocal immunofluorescent analysis of SH-SY5Y cells (left) and HeLa cells (right) using CD56 (NCAM) (123C3) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

NCAM (neural cell adhesion molecule, CD56) is an adhesion glycoprotein with five extracellular immunoglobulin-like domains followed by two fibronectin type III repeats. Structural diversity is introduced by alternative splicing resulting in different cytoplasmic domains (1). NCAM mediates neuronal attachment, neurite extension and cell-cell interactions through homo and heterophilic interactions. PSA (polysialic acid) post-translationally modifies NCAM and increases the metastatic potential of small cell lung carcinoma, Wilms+ tumor, neuroblastoma and rhabdomyosarcoma (2). CD56 and CD16 are commonly used to identify NK cells although some cells with the T cell markers CD3 and CD4 also express CD56 (3).

  1. Cunningham, B. A. et al. (1987) Science 236, 799-806.
  2. Seidenfaden, R. et al. (2003) Mol. Cell. Biol. 23, 5908-5918.
  3. Robertson, M.J. and Ritz, J. (1990) Blood 76, 2421-2438.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products