Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

CD44 Antibody #3578

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 80 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

CD44 Antibody detects endogenous levels of total CD44 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) surrounding Leu135 of human CD44. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from U118MG and Panc1 cells, using CD44 Antibody.

Background

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1-2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3).CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).

  1. Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
  2. Cichy, J. and Pure, E. (2003) J. Cell Biol. 161, 839-843.
  3. Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
  4. Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
  5. Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
  6. Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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