Product Pathways - Wnt / Hedgehog / Notch
Nanog Antibody #3580
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC F | H | Endogenous | 42 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
F=Flow Cytometry
Reactivity Key:
H=Human
Species cross-reactivity is determined by Western blot.
Protocols
- 3580:
- Flow, Immunofluorescence, Western Blotting
Specificity / Sensitivity
Nanog Antibody detects endogenous levels of total nanog protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a syntheitc( peptide corresponding to amino acid sequence at the N-terminus of human nanog. Antibodies are purified by Protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NTERA2 and NCCIT cells using Nanog Antibody.
Flow Cytometry
Flow cytometric analysis of HeLa cells (blue) and NTERA2 cells (green) using Nanog Antibody.
IF-IC
Confocal immunofluorescent analysis of NTERA2 cells (left) and HeLa cells (right) using Nanog Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Background
Nanog is a homeodomain containing transcription factor that is essential for the maintenance of pluripotency and self renewal in embryonic stem cells (1). Nanog expression is controlled by a network of factors including Sox2 and the key pluripotency regulator Oct-4 (1). Recent advances in somatic cell reprogramming have utilized viral expression of combinations of transcription factors including nanog, Oct-4, Sox2, KLF4, c-Myc and Lin28 (2,3).
- Kim, J. et al. (2008) Cell 132, 1049-61.
- Takahashi, K. et al. (2007) Nat Protoc 2, 3081-9.
- Yu, J. et al. (2007) Science 318, 1917-20.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3978 LIN28A (A177) Antibody
- 3979 LIN28A (P22) Antibody
- 2840 Oct-4A (C30A3) Rabbit mAb
- 2890 Oct-4A (C52G3) Rabbit mAb
- 2788 Oct-4 (V241) Antibody
- 2750 Oct-4 Antibody
- 3579 Sox2 (D6D9) XP™ Rabbit mAb
- 2748 Sox2 Antibody
- 2883 TCF3 (D15G11) XP™ Rabbit mAb
- 4904 Stat3 (79D7) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
