Product Pathways - Ca / cAMP / Lipid Signaling
PDE5 (P434) Antibody #3585
PhosphoSitePlus® protein, site, and accession data: PDE5A
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H (Mk) | Endogenous | 100 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
PDE5 (P434) Antibody detects endogenous levels of total PDE5 protein. The antibody cross-reacts with a 35-40 kDa protein of unknown origin.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to central residues of human PDE5.
Western Blotting
Western blot analysis of extracts from LN-18 cells using PDE5 (P434) Antibody.
IP
Immunoprecipitation of PDE5 from LN-18 cells using PDE5 (P434) Antibody followed by western blot using the same antibody. Lane 1 is 5% input.
Background
The superfamily of phosphodiesterases (PDEs) catalyses the hydrolysis of 3',5'-cyclic nucleotides into the corresponding nucleotide 5'-monophosphates. PDE5 is a cGMP-specific enzyme that contains two cGMP binding sites in its amino-terminal regulatory domain (1). Elevation of cGMP levels causes activation of PKG, which phosphorylates PDE5 at Ser102 (2). Phosphorylation of PDE5 stimulates its enzymatic activity and enhances cGMP binding affinity in the regulatory domain, leading to a decrease in intracellular cGMP levels (3,4).
- Corbin, J.D. and Francis, S.H. (1999) J. Biol. Chem. 274, 13729-13732.
- Thomas, L.K. et al. (1990) J. Biol. Chem. 265, 14971-14978.
- Rybalkin, S.D. et al. (2002) J. Biol. Chem. 277, 3310-3317.
- Francis, S.H. et al. (2002) J. Biol. Chem. 277, 47581-47587.
Application References
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Companion Products
- 2395 PDE5 Antibody
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- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
