Cell Signaling Technology

Product Pathways - Translational Control

eEF1A (D10A5) Rabbit mAb #3586

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 50 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eEF1A (D10A5) Rabbit mAb detects endogenous levels of total eEF1A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eEF1A.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using eEF1A (D10A5) Rabbit mAb.

Background

Translation is the process where amino acid residues are assembled into polypeptides on ribosomes. This process is generally divided into three stages: initiation, elongation and termination. During elongation, mRNA and tRNA pair at the two active sites (A and P sites) on the ribosome. A number of eukaryotic elongation factors (eEFs) are involved in this process in mammalian cells (1). eEF1A, also called elongation factor Tu (EF-Tu), binds GTP and interacts with amino acyl-tRNAs to promote recruitment of amino acyl-tRNAs to the A-site of the ribosome (1). After GTP hydrolysis, GDP-eEF1A leaves the ribosome and is later converted back to the GTP-eEF1A by eEF1B (1). Studies have shown that eEF1A is phosphorylated under certain conditions, indicating that its activity is regulated at the post-translational level (2,3).

  1. Browne, G.J. and Proud, C.G. (2002) Eur J Biochem 269, 5360-8.
  2. Venema, R.C. et al. (1991) J Biol Chem 266, 12574-80.
  3. Venema, R.C. et al. (1991) J Biol Chem 266, 11993-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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