Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

BLNK Antibody #3587

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 68, 70 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

BLNK antibody detects endogenous levels of total BLNK protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro48 of human BLNK. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from Raji, BaF3 and RBL-1 cells using BLNK Antibody.

Background

BLNK (B cell linker protein), also known as SLP-65 or BASH, is an adaptor molecule that plays key roles in B cell activation and B cell antigen receptor (BCR) engagement. BLNK acts on the interface between BCR-associated Syk and the downstream signaling cascades (1,2). BLNK has multiple SH2 binding motifs (YXXP) at its amino-terminus and a SH2 domain at its carboxy-terminus. After BCR ligation, BLNK is phosphorylated by Syk at multiple YXXP motifs including Tyr72, Tyr84, Tyr96 and Tyr178 (1). These phosphorylated motifs provide docking sites for signaling molecules such as Btk, PLCgamma and Vav. These signaling molecules bind to BLNK through their SH2 domains and together activate downstream signaling pathways (3,4). Through its SH2 domain, BLNK can also interact with tyrosine-phosphorylated targets such as HPK1 to recruit it to the BCR complex for signaling (5).

  1. Kurosaki, T. and Tsukada, S. (2000) Immunity 12, 1-5.
  2. Fu, C. et al. (1998) Immunity 9, 93-103.
  3. Ishiai, M. et al. (1999) Immunity 10, 117-125.
  4. Baba, Y. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 2582-2586.
  5. Tsuji, S. et al. (2001) J. Exp. Med. 194, 529-539.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Products